fluoxetin competes with cortisol for binding to human serum albumin

نویسندگان

mostafa rezaei-tavirani islamic azad university, science and research branch, tehran, iran.

roya tadayon proteomics research center, shahid behshti university of medical sciences, tehran iran.

seyed alireza mortazavi department of pharmaceutics, school of pharmacy, shahid beheshti university of medical sciences, tehran, iran.

arvin medhet proteomics research center, shahid behshti university of medical sciences, tehran iran

چکیده

human serum albumin (hsa) is an important protein that carries variety of substances like some hormones and drugs in blood. pharmacological studies of the interaction of many drugs and hsa are reported during several decades, specially recently years. interaction of cortisol and fluoxetine hydrochloride (flx) (as a common anti-stress drug) with hsa (as their carrier in blood) has been studied separately by using different spectroscopic techniques. here, considering the increment of anti-stress drugs consumption, conformational change of hsa in presence of cortisol and flx in 50 mm tris buffer, at ph = 7.5 and 37°c, is investigated via ph meter, uv absorption and fluorescence spectroscopy and circular dichroism methods. ph meter findings indicate that the acid denaturation of hsa in the presence of drug and cortisol occurs in the similar manner and this pattern is different relative to the denaturation of hsa in the absence of two reagents. the results of the other techniques consistent with the ph meter findings show that flx effects on the physiochemical properties of hsa are as that of cortisol. in-vivo study in rats confirms in-vitro findings which means blood cortisol level increased in the presence of flx. experimental results indicate that flx and cortisol alter the structural aspects of hsa in similar manner, so, this findings lead to the following reasonable conclusion: “flx is a competitive ligand for the binding of cortisol to hsa. binding of flx to hsa interferes to the interaction of cortisol-hsa.”

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Fluoxetin Competes with Cortisol for Binding to Human Serum Albumin

Human serum albumin (HSA) is an important protein that carries variety of substances like some hormones and drugs in blood. Pharmacological studies of the interaction of many drugs and HSA are reported during several decades, specially recently years. Interaction of cortisol and fluoxetine hydrochloride (FLX) (as a common anti-stress drug) with HSA (as their carrier in blood) has been studied s...

متن کامل

Fluoxetin Competes with Cortisol for Binding to Human Serum Albumin

Human serum albumin (HSA) is an important protein that carries variety of substances like some hormones and drugs in blood. Pharmacological studies of the interaction of many drugs and HSA are reported during several decades, specially recently years. Interaction of cortisol and fluoxetine hydrochloride (FLX) (as a common anti-stress drug) with HSA (as their carrier in blood) has been studied s...

متن کامل

Fluoxetine Competes with Cortisol for Binding to Human Serum Albumin

Human serum albumin (HSA) is an important protein that carries variety of substances like some hormones and drugs in blood. Pharmacological studies of the interaction of many drugs and HSA are reported during several decades, specially recently years. Interaction of cortisol and fluoxetine hydrochloride (FLX) (as a common anti-stress drug) with HSA (as their carrier in blood) has been studied s...

متن کامل

Thermodynamic Analysis for Cationic Surfactants Binding to Bovine Serum Albumin

In the present study, the binding isotherms for interaction of a homologous series of n-alkyltrimethyl ammonium bromides with bovine serum albumin (BSA) have been analyzed on basis of intrinsic thermodynamic quantities. In this regards, the intrinsic Gibbs free energy of binding, AGb(i,)„ has been estimated at various surfactant concentrations and its trend of variation for both binding sets ha...

متن کامل

Probing the Binding of Valacyclovir Hydrochloride to the Human Serum Albumin

UV-visible and Fluorescence spectroscopic methods were employed to study the interaction of human serum albumin (HSA) with Valacyclovir Hydrochloride. Additionally, molecular dynamics and molecular docking simulations were used to visualize and specify the binding site of Valacyclovir Hydrochloride. The Stern-Volmer and van't Hoff equations along with spectroscopic observations, were used to de...

متن کامل

Comparative Binding Affinities of Flavonoid Phytochemicals with Bovine Serum Albumin

Dietary flavonoids show beneficial effects in the prevention of chronic diseases. However, flavonoid bioavailability is poor, probably due to their interaction with serum albumins. In the current work, the binding interactions of eight related flavonoids, sharing a similar core structure, with bovine serum albumin (BSA) were investigated by fluorescence spectroscopy. The binding affinities of t...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of pharmaceutical research

جلد ۱۱، شماره ۱، صفحات ۳۲۵-۳۳۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023